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anti nfκb p50  (Santa Cruz Biotechnology)
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The expression of mRNAs for nuclear factor (NF)κB1 <t>(p50),</t> NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.
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proteins map  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology proteins map
The expression of mRNAs for nuclear factor (NF)κB1 <t>(p50),</t> NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.
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pvp/pva hydrogel-forming maps  (Dawley Inc)
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The expression of mRNAs for nuclear factor (NF)κB1 <t>(p50),</t> NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.
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spm 12.0  (SPSS Inc)
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SPSS Inc spm 12.0
The expression of mRNAs for nuclear factor (NF)κB1 <t>(p50),</t> NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.
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control allele frequencies  (Perlegen Sciences)
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Perlegen Sciences control allele frequencies
The expression of mRNAs for nuclear factor (NF)κB1 <t>(p50),</t> NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.
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photoimpact software version 12.0  (Ulead Systems Inc)
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Ulead Systems Inc photoimpact software version 12.0
The expression of mRNAs for nuclear factor (NF)κB1 <t>(p50),</t> NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.
Photoimpact Software Version 12.0, supplied by Ulead Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The expression of mRNAs for nuclear factor (NF)κB1 (p50), NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.

Journal: Arthritis Research & Therapy

Article Title: Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis

doi: 10.1186/ar1915

Figure Lengend Snippet: The expression of mRNAs for nuclear factor (NF)κB1 (p50), NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Total RNA was isolated from purified bone marrow CD34+ cells. The expression of mRNAs for NFκB1, NFκB2, RelA and β-actin was evaluated by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. Horizontal lines indicate the mean values. Statistical significance was evaluated by Welch's t test. OA, osteoarthritis; RA, rheumatoid arthritis.

Article Snippet: Purified bone marrow CD34+ cells (obtained from three RA patients and three OA patients) were treated with IntraPrepTM Permeabilization Reagent (Immunotech, Marseille, France), followed by staining with phycoerythrin (PE)-conjugated anti-NFκB p50 (E-10; a mouse IgG1 monoclonal antibody against amino acids 120 to 239 mapping at the amino terminus of human NFκB p50; Santa Cruz Biotech, Santa Cruz, CA, USA) or PE-conjugated normal mouse IgG1 (Santa Cruz).

Techniques: Expressing, Isolation, Purification, Real-time Polymerase Chain Reaction

Expression of nuclear factor (NF)κB1 (p50) protein in bone marrow CD34+ cells. Purified bone marrow CD34+ cells from a rheumatoid arthritis patient were permeabilized and then stained with phychoerythrin-conjugated anti-NFκB p50 monoclonal antibody or phychoerythrin-conjugated normal mouse IgG1, followed by analysis with flow cytometry. The level of NFκB1 protein was expressed by mean fluorescence intensity as described in Materials and methods.

Journal: Arthritis Research & Therapy

Article Title: Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis

doi: 10.1186/ar1915

Figure Lengend Snippet: Expression of nuclear factor (NF)κB1 (p50) protein in bone marrow CD34+ cells. Purified bone marrow CD34+ cells from a rheumatoid arthritis patient were permeabilized and then stained with phychoerythrin-conjugated anti-NFκB p50 monoclonal antibody or phychoerythrin-conjugated normal mouse IgG1, followed by analysis with flow cytometry. The level of NFκB1 protein was expressed by mean fluorescence intensity as described in Materials and methods.

Article Snippet: Purified bone marrow CD34+ cells (obtained from three RA patients and three OA patients) were treated with IntraPrepTM Permeabilization Reagent (Immunotech, Marseille, France), followed by staining with phycoerythrin (PE)-conjugated anti-NFκB p50 (E-10; a mouse IgG1 monoclonal antibody against amino acids 120 to 239 mapping at the amino terminus of human NFκB p50; Santa Cruz Biotech, Santa Cruz, CA, USA) or PE-conjugated normal mouse IgG1 (Santa Cruz).

Techniques: Expressing, Purification, Staining, Flow Cytometry, Fluorescence

Comparison of the expression of nuclear factor (NF)κB1 (p50) protein with that of NFκB1 mRNA in bone marrow CD34+ cells. Purified bone marrow CD34+ cells were permeabilized and then stained with phychoerythrin-conjugated anti-NFκB p50 monoclonal antibody or phychoerythrin-conjugated normal mouse IgG1, followed by analysis with flow cytometry. The NFκB1 protein levels as expressed by mean fluorescence intensity were compared with NFκB1 mRNA levels (expressed as the ratio of the mRNA copy numbers to those of β-actin) in bone marrow CD34+ cells from six patients (three rheumatoid arthritis patients and three osteoarthritis patients). Statistical significance was evaluated by linear regression test.

Journal: Arthritis Research & Therapy

Article Title: Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis

doi: 10.1186/ar1915

Figure Lengend Snippet: Comparison of the expression of nuclear factor (NF)κB1 (p50) protein with that of NFκB1 mRNA in bone marrow CD34+ cells. Purified bone marrow CD34+ cells were permeabilized and then stained with phychoerythrin-conjugated anti-NFκB p50 monoclonal antibody or phychoerythrin-conjugated normal mouse IgG1, followed by analysis with flow cytometry. The NFκB1 protein levels as expressed by mean fluorescence intensity were compared with NFκB1 mRNA levels (expressed as the ratio of the mRNA copy numbers to those of β-actin) in bone marrow CD34+ cells from six patients (three rheumatoid arthritis patients and three osteoarthritis patients). Statistical significance was evaluated by linear regression test.

Article Snippet: Purified bone marrow CD34+ cells (obtained from three RA patients and three OA patients) were treated with IntraPrepTM Permeabilization Reagent (Immunotech, Marseille, France), followed by staining with phycoerythrin (PE)-conjugated anti-NFκB p50 (E-10; a mouse IgG1 monoclonal antibody against amino acids 120 to 239 mapping at the amino terminus of human NFκB p50; Santa Cruz Biotech, Santa Cruz, CA, USA) or PE-conjugated normal mouse IgG1 (Santa Cruz).

Techniques: Comparison, Expressing, Purification, Staining, Flow Cytometry, Fluorescence

Comparison of the expression of nuclear factor (NF)κB1 (p50) mRNA in bone marrow CD34+ cells with their capacity to differentiate into fibroblast-like cells. The expression of NFκB1 mRNA in bone marrow CD34+ cells from 12 rheumatoid arthritis patients was evaluated by real-time quantitative PCR prior to the culture. The bone marrow CD34+ cells were incubated in culture medium with stem cell factor (10 ng/ml), granulocyte-macrophage colony stimulating factor (1 ng/ml) and tumor necrosis factor-α (10 ng/ml) for 4 weeks with no medium changes. Morphological changes were evaluated under light microscopy. The percentages of fibroblast-like cells were calculated from two view fields at ×20 magnifications. The degree of the generation of fibroblast-like cells were scored as follows: 0, fibroblast-like cells <5%; 1, fibroblast-like cells 5% to 25%; 2, fibroblast-like cells 25% to 50%; 3, fibroblast-like cells >50%; 4, formation of a pile or a cluster in at least one view field. Statistical significance was evaluated by Spearman's rank correlation test.

Journal: Arthritis Research & Therapy

Article Title: Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis

doi: 10.1186/ar1915

Figure Lengend Snippet: Comparison of the expression of nuclear factor (NF)κB1 (p50) mRNA in bone marrow CD34+ cells with their capacity to differentiate into fibroblast-like cells. The expression of NFκB1 mRNA in bone marrow CD34+ cells from 12 rheumatoid arthritis patients was evaluated by real-time quantitative PCR prior to the culture. The bone marrow CD34+ cells were incubated in culture medium with stem cell factor (10 ng/ml), granulocyte-macrophage colony stimulating factor (1 ng/ml) and tumor necrosis factor-α (10 ng/ml) for 4 weeks with no medium changes. Morphological changes were evaluated under light microscopy. The percentages of fibroblast-like cells were calculated from two view fields at ×20 magnifications. The degree of the generation of fibroblast-like cells were scored as follows: 0, fibroblast-like cells <5%; 1, fibroblast-like cells 5% to 25%; 2, fibroblast-like cells 25% to 50%; 3, fibroblast-like cells >50%; 4, formation of a pile or a cluster in at least one view field. Statistical significance was evaluated by Spearman's rank correlation test.

Article Snippet: Purified bone marrow CD34+ cells (obtained from three RA patients and three OA patients) were treated with IntraPrepTM Permeabilization Reagent (Immunotech, Marseille, France), followed by staining with phycoerythrin (PE)-conjugated anti-NFκB p50 (E-10; a mouse IgG1 monoclonal antibody against amino acids 120 to 239 mapping at the amino terminus of human NFκB p50; Santa Cruz Biotech, Santa Cruz, CA, USA) or PE-conjugated normal mouse IgG1 (Santa Cruz).

Techniques: Comparison, Expressing, Real-time Polymerase Chain Reaction, Incubation, Light Microscopy

Effect of tumor necrosis factor (TNF)-α on the expression of mRNAs for nuclear factor (NF)κB1 (p50), NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Bone marrow CD34+ cells from healthy individuals were incubated in culture medium with or without TNF-α (10 ng/ml) for 24 hours. After the incubation, total RNA was isolated for evaluation of the expression of mRNAs for NFκB1, NFκB2, RelA and β-actin by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. The data are representative of two different experiments.

Journal: Arthritis Research & Therapy

Article Title: Enhanced expression of mRNA for nuclear factor κB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis

doi: 10.1186/ar1915

Figure Lengend Snippet: Effect of tumor necrosis factor (TNF)-α on the expression of mRNAs for nuclear factor (NF)κB1 (p50), NFκB2 (p52) and RelA (p65) in bone marrow CD34+ cells. Bone marrow CD34+ cells from healthy individuals were incubated in culture medium with or without TNF-α (10 ng/ml) for 24 hours. After the incubation, total RNA was isolated for evaluation of the expression of mRNAs for NFκB1, NFκB2, RelA and β-actin by real-time quantitative PCR. The data are expressed as the ratio of the mRNA copy numbers to those of β-actin. The data are representative of two different experiments.

Article Snippet: Purified bone marrow CD34+ cells (obtained from three RA patients and three OA patients) were treated with IntraPrepTM Permeabilization Reagent (Immunotech, Marseille, France), followed by staining with phycoerythrin (PE)-conjugated anti-NFκB p50 (E-10; a mouse IgG1 monoclonal antibody against amino acids 120 to 239 mapping at the amino terminus of human NFκB p50; Santa Cruz Biotech, Santa Cruz, CA, USA) or PE-conjugated normal mouse IgG1 (Santa Cruz).

Techniques: Expressing, Incubation, Isolation, Real-time Polymerase Chain Reaction